The concepts of biochemical genetics have already been, and will undoubtedly continue to be, significant in broader areas of biology.

This was before the universality of need for the B vitamins, and the enzymatic basis of this requirement, had been clearly defined.

In microbiology the roles of mutation and selection in evolution are coming to be better understood through the use of bacterial cultures of mutant strains.

As has repeatedly been stated, the underlying hypothesis, which in a number of cases has been supported by direct experimental evidence, is that each gene controls the production, function, and specificity of a particular enzyme.

That the primary effect of gene mutation may be as simple as the substitution of a single amino acid by another and may lead to profound secondary changes in protein structure and properties has recently been strongly indicated by the work of Ingram on hemoglobin.